C1D Polyklonaler Antikörper

C1D Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 10711-1-AP

Synonyme

C1D, hC1D, LRP1, SUN CoR, SUNCOR

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• 

  WB analysis of DU 145 using 10711-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10711-1-AP (C1D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of DU 145 using 10711-1-AP

  IP result of anti-C1D (IP:10711-1-AP, 4ug; Detection:10711-1-AP 1:300) with DU 145 cells lysate 1800 ug.

• 

  IHC staining of human prostate cancer using 10711-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10711-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10711-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10711-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10711-1-AP (C1D Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate cancer using 10711-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10711-1-AP (C1D antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen
• Erfolgreiche IP: DU 145-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10711-1-AP bindet in WB, IP, IHC, ELISA C1D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C1D fusion protein Ag1121
• Vollständiger Name: C1D nuclear receptor co-repressor
• Berechnetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC005235
• Gene symbol: C1D
• Gene ID (NCBI): 10438
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; Multi-omics profiling reveals Chitinase-3-like protein 1 as a key mediator in the crosstalk between sarcopenia and liver cancer; Authors - Di Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ralf (Verified Customer) (08-04-2022)

10µg of total mouse protein were loaded on the gel. Antibodies also stain the ladder.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200

C1D Antibody Western Blot validation (1:200 dilution) in (Cat no:10711-1-AP)