C1QA Monoklonaler Antikörper

C1QA Monoklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1B10E3

Kat-Nr. : 67063-1-Ig

Synonyme

C1QA

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 67063-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 67063-1-Ig

  human blood were subjected to SDS PAGE followed by western blot with 67063-1-Ig (C1QA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67063-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67063-1-Ig (C1QA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67063-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C1QA antibody (67063-1-Ig, Clone: 1B10E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, humanes Blutgewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

67063-1-Ig bindet in WB, IHC, IF, ELISA C1QA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C1QA fusion protein Ag28622
• Vollständiger Name: complement component 1, q subcomponent, A chain
• Berechnetes Molekulargewicht: 245 aa, 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC030153
• Gene symbol: C1QA
• Gene ID (NCBI): 712
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Publikationen

Species	Application	Title
human	WB	Onco Targets Ther; Prognostic Implications of the Complement Protein C1Q and Its Correlation with Immune Infiltrates in Osteosarcoma.; Authors - Hanji Huang
human	IF	Cells; Human Placental Endothelial Cell and Trophoblast Heterogeneity and Differentiation Revealed by Single-Cell RNA Sequencing; Authors - Han Li
human	IF	Front Pharmacol; The proportion of C1q-high and ISG15-high monocytes in the skin of patients with Behçet disease; Authors - Yangtengyu Liu