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C1QA Monoklonaler Antikörper

C1QA Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1B10E3

Kat-Nr. : CL594-67063

Synonyme

C1QA

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated C1QA Monoclonal antibody (CL594-67063)

    IF Staining of HepG2 using CL594-67063

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 C1QA antibody (CL594-67063, Clone: 1B10E3 ) at dilution of 1:200, CL488-Phalloidin (green).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67063 bindet in IF/ICC C1QA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen C1QA fusion protein Ag28622
Vollständiger Name complement component 1, q subcomponent, A chain
Berechnetes Molekulargewicht 245 aa, 26 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC030153
Gene symbol C1QA
Gene ID (NCBI) 712
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qA which is the A-chain polypeptide of human complement subcomponent C1q.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 C1QA antibody CL594-67063Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen