C1qC Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C1qC Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16889-1-AP

Synonyme

C1Q C, Complement C1q C chain, C1QG

---

• 

  WB analysis using 16889-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse serum using 16889-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16889-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 16889-1-AP (C1qC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16889-1-AP (C1QC Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16889-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16889-1-AP (C1QC Antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, mouse serum, rat plasma
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

16889-1-AP bindet in WB, IHC, IF, ELISA C1qC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C1qC fusion protein Ag10400
• Vollständiger Name: complement component 1, q subcomponent, C chain
• Berechnetes Molekulargewicht: 245 aa, 26 kDa
• Beobachtetes Molekulargewicht: 24-26 kDa
• GenBank-Zugangsnummer: BC009016
• Gene symbol: C1QC
• Gene ID (NCBI): 714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.

Publikationen

Species	Application	Title
human	WB	World J Surg Oncol; Proteomic analysis of small extracellular vesicles from the plasma of patients with hepatocellular carcinoma; Authors - Wei Dong
human	WB	Front Genet; C1QC is a prognostic biomarker with immune-related value in kidney renal clear cell carcinoma; Authors - Wentao Yao ; KD Validated
human	IHC	J Cancer; Identification of crucial genes through WGCNA in the progression of gastric cancer; Authors - Rui Liu
mouse	WB,IF	Mol Neurobiol; Upregulation of C1QC as a Mediator of Blood-Brain Barrier Damage in Type 2 Diabetes Mellitus; Authors - Cheng Huang
human	IHC	Sci Rep; Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies; Authors - Yang Yang