C1qC Rekombinanter Antikörper

C1qC Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242624B1

Kat-Nr. : 85031-5-PBS

Synonyme

Complement C1q C chain, C1Q C, 242624B1, C1QG

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  WB analysis of human plasma using 85031-5-RR (same clone as 85031-5-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 85031-5-RR (C1QC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85031-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 85031-5-RR (same clone as 85031-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85031-5-RR (C1qC antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85031-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85031-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85031-5-RR against Human C1qC were performed. The affinity constant is 1.26 nM.

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Geprüfte Anwendungen

Produktinformation

85031-5-PBS bindet in WB, IHC, Indirect ELISA C1qC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: C1qC fusion protein Ag10400
• Vollständiger Name: complement component 1, q subcomponent, C chain
• Berechnetes Molekulargewicht: 245 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC009016
• Gene symbol: C1QC
• Gene ID (NCBI): 714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first component of complement, C1, is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. This antibody is raised against C1qC which is the C-chain polypeptide of human complement subcomponent C1q.