C1r Polyklonaler Antikörper

C1r Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17346-1-AP

Synonyme

Complement C1r, Complement C1r subcomponent, Complement C1r subcomponent heavy chain, Complement C1r subcomponent light chain, EC:3.4.21.41

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  WB analysis using 17346-1-AP

  human plasma was subjected to SDS PAGE followed by western blot with 17346-1-AP (C1r antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 17346-1-AP

  Human blood was subjected to SDS PAGE, followed by western blot with 17346-1-AP (C1r Antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17346-1-AP (C1r Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 17346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17346-1-AP (C1R Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, human blood
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

17346-1-AP bindet in WB, IHC, IP, ELISA C1r und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C1r fusion protein Ag11390
• Vollständiger Name: complement component 1, r subcomponent
• Berechnetes Molekulargewicht: 705 aa, 80 kDa
• Beobachtetes Molekulargewicht: 85 kDa, 30 kDa
• GenBank-Zugangsnummer: BC035220
• Gene symbol: C1R
• Gene ID (NCBI): 715
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The first component of complement is a calcium-dependent complex of the 3 subcomponents C1q, C1r, and C1s. The serine protease subunits C1r and C1s form a Ca(2+)-dependent C1s-C1r-C1r-C1s tetramer. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein that can be cleaved into an A chain and a B chain upon activation. This antibody recognizes the full-length C1r chain and the cleaved B chain.

Publikationen

Species	Application	Title
mouse,human	WB,IP,IHC	Mol Carcinog; Downregulation of C1R promotes hepatocellular carcinoma development by activating HIF-1α-regulated glycolysis; Authors - Yuying Ma
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts