C1orf58 Polyklonaler Antikörper

C1orf58 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26109-1-PBS

Synonyme

BROX, BROFTI, BRO1 domain-containing protein BROX, BRO1 domain containing protein BROX, BRO1 domain- and CAAX motif-containing protein

---

• 

  WB analysis using 26109-1-AP (same clone as 26109-1-PBS)

  HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 26109-1-AP (same clone as 26109-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26109-1-AP (C1orf58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 26109-1-AP (same clone as 26109-1-PBS)

  IP result of anti-C1orf58 (IP:26109-1-AP, 4ug; Detection:26109-1-AP 1:300) with HepG2 cells lysate 1680 ug. This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 26109-1-AP (same clone as 26109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 26109-1-AP (same clone as 26109-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26109-1-AP (C1orf58 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 26109-1-AP (same clone as 26109-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf58 antibody (26109-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 26109-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

26109-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA C1orf58 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C1orf58 fusion protein Ag23443
• Vollständiger Name: chromosome 1 open reading frame 58
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC113635
• Gene symbol: C1orf58
• Gene ID (NCBI): 148362
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.