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C1orf77 Rekombinanter Antikörper

C1orf77 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242770H3

Kat-Nr. : 85147-3-PBS

Synonyme

CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704

Filter:
  • Western Blot (WB) analysis of various lysates using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    WB analysis using 85147-3-RR (same clone as 85147-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85147-3-RR (C1orf77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    IHC staining of human colon cancer using 85147-3-RR (same clone as 85147-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    IHC staining of human colon cancer using 85147-3-RR (same clone as 85147-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    IHC staining of human stomach using 85147-3-RR (same clone as 85147-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    IF Staining of HeLa using 85147-3-RR (same clone as 85147-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    IF Staining of SH-SY5Y using 85147-3-RR (same clone as 85147-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using C1orf77 Recombinant monoclonal antibody (85147-3-RR)

    IF Staining of A431 using 85147-3-RR (same clone as 85147-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85147-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01863-1

    Cytometric bead array standard curve of MP01863-1

    Cytometric bead array standard curve of MP01863-1, C1orf77 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85147-3-PBS. Detection antibody: 85147-1-PBS. Standard: Ag26580. Range: 1.563-200 ng/mL.

  • Affinity and Kinetic Characterization of 85147-3-RR

    Affinity and Kinetic Characterization of 85147-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85147-3-RR against Human C1orf77 were performed. The affinity constant is 9.63 nM.

Geprüfte Anwendungen

Produktinformation

85147-3-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA C1orf77 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen C1orf77 fusion protein Ag26580
Vollständiger Name chromosome 1 open reading frame 77
Berechnetes Molekulargewicht 248 aa, 26 kDa
Beobachtetes Molekulargewicht26-30 kDa
GenBank-ZugangsnummerBC002733
Gene symbol C1orf77
Gene ID (NCBI) 26097
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.