C1orf77 Rekombinanter Antikörper

C1orf77 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242770H3

Kat-Nr. : 85147-3-RR

Synonyme

CHTOP, FOP, 242770H3, Friend of PRMT1 protein, PP7704

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  WB analysis using 85147-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85147-3-RR (C1orf77 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85147-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85147-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 85147-3-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 85147-3-RR (C1orf77 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 85147-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of SH-SY5Y using 85147-3-RR

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using 85147-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf77 antibody (85147-3-RR, Clone: 242770H3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85147-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85147-3-RR against Human C1orf77 were performed. The affinity constant is 9.63 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Jurkat-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen, A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85147-3-RR bindet in WB, IHC, IF/ICC, ELISA C1orf77 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: C1orf77 fusion protein Ag26580
• Vollständiger Name: chromosome 1 open reading frame 77
• Berechnetes Molekulargewicht: 248 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC002733
• Gene symbol: C1orf77
• Gene ID (NCBI): 26097
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.