C1orf83 Polyklonaler Antikörper

C1orf83 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20940-1-AP

Synonyme

FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2, Transcription elongation factor A N-terminal and central domain-containing protein 2

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  WB analysis using 20940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20940-1-AP (C1orf83 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20940-1-AP (C1orf83 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 20940-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 20940-1-AP (C1orf83 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 20940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C1orf83 antibody (20940-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Hirngewebe, humanes Mammakarzinomgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20940-1-AP bindet in WB, IHC, IF/ICC, ELISA C1orf83 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C1orf83 fusion protein Ag15040
• Vollständiger Name: chromosome 1 open reading frame 83
• Berechnetes Molekulargewicht: 208 aa, 23 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC035374
• Gene symbol: C1orf83
• Gene ID (NCBI): 127428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromosome 1 open reading frame 83 (C1orf83) is also called TCEANC2, a little-studied transcription elongation factor. The rs10788972 variant of the TCEANC2 gene in the PARK10 region is closely associated with sporadic PD in the American population, while both rs10788972 and rs12046178 variants are not associated with sporadic PD in the Chinese Han population (PMID: 25663231; PMID: 26432391).