C1orf83 Rekombinanter Antikörper

C1orf83 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240678D10

Kat-Nr. : 83626-3-PBS

Synonyme

240678D10, FLJ32112, FLJ39169, RP4 758J24.3, TCEANC2

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  IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 83626-3-RR (same clone as 83626-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 83626-3-RR (C1orf83 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83626-3-RR (same clone as 83626-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf83 antibody (83626-3-RR, Clone: 240678D10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83626-3-RR (same clone as 83626-3-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug C1orf83 Recombinant antibody (83626-3-RR, Clone:240678D10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83626-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00578-2

  Cytometric bead array standard curve of MP00578-2, C1orf83 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83626-1-PBS. Detection antibody: 83626-3-PBS. Standard: Ag15040. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83626-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83626-3-RR against Human C1orf83 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83626-3-PBS bindet in IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA C1orf83 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: C1orf83 fusion protein Ag15040
• Vollständiger Name: chromosome 1 open reading frame 83
• Berechnetes Molekulargewicht: 208 aa, 23 kDa
• GenBank-Zugangsnummer: BC035374
• Gene symbol: C1orf83
• Gene ID (NCBI): 127428
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.