Complement C2 Polyklonaler Antikörper

Complement C2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27175-1-AP

Synonyme

C2, complement component 2, Complement C2b fragment, Complement C2a fragment, CO2

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  WB analysis of A431 using 27175-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27175-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27175-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27175-1-AP (C2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27175-1-AP (C2 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HepG2-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

27175-1-AP bindet in WB, IHC, ELISA Complement C2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Complement C2 fusion protein Ag25690
• Vollständiger Name: complement component 2
• Beobachtetes Molekulargewicht: 70 kDa, 80 kDa
• GenBank-Zugangsnummer: BC043484
• Gene symbol: C2
• Gene ID (NCBI): 717
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement C2 (C2) is a key component of the classical and lectin pathways of the complement system. Complement C2 deficiency is associated with an increased susceptibility to infections, particularly those caused by encapsulated bacteria.

Publikationen

Species	Application	Title
human	WB	Cell Mol Gastroenterol Hepatol; A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.; Authors - Dayan Sun
	IHC	Insect Biochem Mol Biol; Sequence analysis and function of mosquito aeCCC2 and Drosophila Ncc83 orthologs.; Authors - Phu C Duong