GID8 Polyklonaler Antikörper

GID8 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Rezensionen (1)

Kat-Nr. : 24479-1-AP

Synonyme

C20orf11, Glucose-induced degradation protein 8 homolog, Protein C20orf11, Twa1, Two hybrid-associated protein 1 with RanBPM

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  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24479-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24479-1-AP (GID8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 24479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24479-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24479-1-AP (GID8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, Rattenhirngewebe, SW480-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

24479-1-AP bindet in WB, IHC, IF, ELISA GID8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GID8 fusion protein Ag19751
• Vollständiger Name: chromosome 20 open reading frame 11
• Berechnetes Molekulargewicht: 228 aa, 27 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC032120
• Gene symbol: GID8
• Gene ID (NCBI): 54994
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The GID8 protein is a component of the Glucose-Induced Degradation-deficient (GID) complex, which plays a key role in ubiquitin-mediated protein degradation, a pathway used to regulate protein turnover in eukaryotic cells. Initially discovered in yeast, GID8 acts as a scaffold protein that is essential for the assembly of the multi-subunit GID complex and for substrate recognition. This complex plays a significant role in metabolic regulation, such as degrading fructose-1,6-bisphosphatase (FBPase) during the shift from gluconeogenesis to glycolysis. In humans, GID8 is part of the CTLH complex, which is associated with various cellular processes including cell cycle regulation, apoptosis, and cancer progression.

Publikationen

Species	Application	Title
human	WB	PLoS Genet; Role of Bicaudal C1 in renal gluconeogenesis and its novel interaction with the CTLH complex.; Authors - Lucia Carolina Leal-Esteban
human	WB	Sci Rep; Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin.; Authors - Shimaa H A Soliman
human	WB	bioRxiv; Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth; Authors - Nelson V Simwela
human	WB,IHC,IF	Cancer Lett; YTHDF1 regulates GID8-mediated glutamine metabolism to promote colorectal cancer progression in a m6A-dependent manner; Authors - Yicun Han
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi
mouse	WB	Nat Commun; Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth; Authors - Nelson V Simwela

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

GID8 was pulled down with MAEA-flag, so I could tell which band is GID8. But without IP, it would be difficult to tell which band is the GID8, because the on-target band signal is too weak, while off-target band signals are strong in HEK293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

GID8 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:24479-1-AP)