PPDPF Polyklonaler Antikörper

PPDPF Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 19912-1-AP

Synonyme

C20orf149, EXPDF

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  WB analysis using 19912-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 19912-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19912-1-AP (C20orf149 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 19912-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of U2OS using 19912-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPDPF antibody (19912-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Produktinformation

19912-1-AP bindet in WB, IHC, IF/ICC, ELISA PPDPF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPDPF fusion protein Ag13740
• Vollständiger Name: chromosome 20 open reading frame 149
• Berechnetes Molekulargewicht: 114 aa, 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC002531
• Gene symbol: PPDPF
• Gene ID (NCBI): 79144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF), also known as C20orf149 and EXPDF, belongs to the PPDPF family. PPDPF is a key regulator for the development of exocrine pancreas. PPDPF is highly expressed in various cancers, including HCC, colorectal cancer, prostate cancer, etc (PMID: 34031390, 34975328, 37027301). PPDPF consists of 114 amino acids and the molecular weight is 12 kDa.

Publikationen

Species	Application	Title
		Cell Metab; Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.; Authors - Marcel Morgenstern
mouse,human	WB,IHC	Cell Rep; PPDPF suppresses the development of hepatocellular carcinoma through TRIM21-mediated ubiquitination of RIPK1; Authors - Yi-Kang Wang
human	WB	Int J Biol Sci; PPDPF Promotes the Progression and acts as an Antiapoptotic Protein in Non-Small Cell Lung Cancer.; Authors - Mu Yun
human	WB	Front Oncol; The Exocrine Differentiation and Proliferation Factor (EXDPF) Gene Promotes Ovarian Cancer Tumorigenesis by Up-Regulating DNA Replication Pathway.; Authors - Yangjiong Xiao
human	WB,IHC	Medicine (Baltimore); Pancreatic progenitor cell differentiation and proliferation factor predicts poor prognosis in heptaocellular carcinoma.; Authors - Zhengfa Mao
human	WB,IHC,IF	EMBO Rep; Phosphorylation of PPDPF via IL6-JAK2 activates the Wnt/β-catenin pathway in colorectal cancer; Authors - Yuyuan Zi