C20orf20 Polyklonaler Antikörper

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• KD/KO Validated

C20orf20 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 26040-1-AP

Synonyme

Eaf7, MRG/MORF4L-binding protein, MRG15BP, MRG-binding protein, MRGBP

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  WB analysis of HepG2 using 26040-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26040-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26040-1-AP (C20orf20 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 26040-1-AP

  IP result of anti-C20orf20 (IP:26040-1-AP, 4ug; Detection:26040-1-AP 1:300) with HepG2 cells lysate 2640ug.

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  IHC staining of human colon cancer using 26040-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 26040-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26040-1-AP (C20orf20 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26040-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C20orf20 antibody (26040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushodengewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

26040-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA C20orf20 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C20orf20 fusion protein Ag22840
• Vollständiger Name: chromosome 20 open reading frame 20
• Berechnetes Molekulargewicht: 204 aa, 22 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC014235
• Gene symbol: C20orf20
• Gene ID (NCBI): 55257
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C20orf20, also named as MRG/MORF4L-binding protein or Up-regulated in colon cancer 4 (Urcc4), is a 204 amino acid protein, which belongs to the EAF7 family. C20orf20 localizes in the nucleus and is a Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. C20orf20 is modified through phosphorylation and acetylation, so the molecular weight of C20orf20 might be 25-30 kDa.

Publikationen

Species	Application	Title
human	WB	Biochim Biophys Acta; MRGBP promotes AR-mediated transactivation of KLK3 and TMPRSS2 via acetylation of histone H2A.Z in prostate cancer cells.; Authors - Saya Ito ; KD Validated
human	IHC	J Cell Mol Med; Pathway-based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis; Authors - Ruoyu Huang
human	WB	Nature; BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network; Authors - Xueqin Sun