C21orf2 Polyklonaler Antikörper

C21orf2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 27609-1-AP

Synonyme

A2, C21orf-HUMF09G8.5, CFAP410, Cilia- and flagella-associated protein 410, Leucine-rich repeat-containing protein 76

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  WB analysis using 27609-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27609-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 27609-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 27609-1-AP (C21orf2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 27609-1-AP

  IP result of anti-C21orf2 (IP:27609-1-AP, 4ug; Detection:27609-1-AP 1:3000) with HEK-293T cells lysate 1320 ug.

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  IHC staining of human lung cancer using 27609-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 27609-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27609-1-AP (C21orf2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen
• Erfolgreiche IP: HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

27609-1-AP bindet in WB, IHC, IP, ELISA C21orf2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C21orf2 fusion protein Ag26470
• Vollständiger Name: chromosome 21 open reading frame 2
• Beobachtetes Molekulargewicht: 28-32 kDa
• GenBank-Zugangsnummer: BC072012
• Gene symbol: C21orf2
• Gene ID (NCBI): 755
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IP	Life Sci Alliance; Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases; Authors - Mateusz Gregorczyk
human	WB	Acta Neuropathol Commun; ALS-associated C21ORF2 variant disrupts DNA damage repair, mitochondrial metabolism, neuronal excitability and NEK1 levels in human motor neurons; Authors - Pavol Zelina