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RTCB Rekombinanter Antikörper

RTCB Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250866B2

Kat-Nr. : 86258-4-PBS

Synonyme

HSPC117, 3'-phosphate/5'-hydroxy nucleic acid ligase, C22orf28, EC:6.5.1.8, FAAP

Filter:
  • Western Blot (WB) analysis of various lysates using RTCB Recombinant monoclonal antibody (86258-4-RR)

    WB analysis using 86258-4-RR (same clone as 86258-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86258-4-RR (C22orf28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86258-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using RTCB Recombinant monoclonal antibody (86258-4-RR)

    IF Staining of Jurkat using 86258-4-RR (same clone as 86258-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RTCB antibody (86258-4-RR, Clone: 250866B2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86258-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86258-4-PBS

    Affinity and Kinetic Characterization of 86258-4-PBS

    Biolayer interferometry (BLl) kinetic assays of 86258-4-PBS against Human RTCB were performed. The affinity constant is 0.303 nM.

Geprüfte Anwendungen

Produktinformation

86258-4-PBS bindet in WB, IF/ICC, Indirect ELISA RTCB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RTCB fusion protein Ag13836
Vollständiger Name chromosome 22 open reading frame 28
Berechnetes Molekulargewicht 55 kDa
Beobachtetes Molekulargewicht55 kDa
GenBank-ZugangsnummerBC000151
Gene symbol RTCB
Gene ID (NCBI) 51493
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RTCB (also known as HSPC117, C22orf28, FAAP and D10Wsu52e) is an essential subunit of a tRNA ligase complex that is involved in RNA repair and stress-induced splicing. As a multifunctional protein, RTCB is also a cell adhesion protein and is important in embryo and placenta development. Recently RTCB has been identified as UPR RNA ligase catalyzing unconventional XBP1 mRNA splicing. This antibody specifically recognizes endogenous RTCB protein. (25087875)