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C3/C3b/C3c Polyklonaler Antikörper
C3/C3b/C3c Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 21337-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | rat plasma, human plasma |
Erfolgreiche IP | humanes Plasmagewebe |
Erfolgreiche Detektion in IHC | humanes Lebergewebe, humanes Leberkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | humanes Leberkarzinomgewebe |
Erfolgreiche Detektion in IF/ICC | HepG2-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:1000-1:4000 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 66 publications below |
IHC | See 15 publications below |
IF | See 54 publications below |
CoIP | See 1 publications below |
Produktinformation
21337-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA C3/C3b/C3c und zeigt Reaktivität mit human, Ratten
Getestete Reaktivität | human, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | C3/C3b/C3c fusion protein Ag15537 |
Vollständiger Name | complement component 3 |
Berechnetes Molekulargewicht | 1663 aa, 187 kDa |
Beobachtetes Molekulargewicht | 115 kDa |
GenBank-Zugangsnummer | BC150179 |
Gene symbol | C3 |
Gene ID (NCBI) | 718 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in activating the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3 (190-195 kDa), composed of α and β chains (115-120 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is composed of the α' chain and β chain (PMID: 27210597). Factor I cleaves the α′ chain of C3b to 68 kDa and 43 kDa degradation products (iC3b) (PMID: 25395424; 14527961). This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 α chain (115-120 kDa), C3b α' chain (110-115 kDa), and C3c α' chain fragment 2 (43 kDa).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for C3/C3b/C3c antibody 21337-1-AP | Protokoll herunterladen |
IHC protocol for C3/C3b/C3c antibody 21337-1-AP | Protokoll herunterladenl |
IF protocol for C3/C3b/C3c antibody 21337-1-AP | Protokoll herunterladen |
IP protocol for C3/C3b/C3c antibody 21337-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nat Cell Biol Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence | ||
Brain Behav Immun PDE4 inhibition alleviates HMGB1/C1q/C3-mediated excessive phagocytic pruning of synapses by microglia and depressive-like behaviors in mice | ||
J Autoimmun TIGIT-Fc fusion protein alleviates murine lupus nephritis through the regulation of SPI-B-PAX5-XBP1 axis-mediated B-cell differentiation | ||
Gut Microbes Targeted modulation of intestinal barrier and mucosal immune-related microbiota attenuates IgA nephropathy progression | ||
Nat Commun Increased circulating levels of Factor H-Related Protein 4 are strongly associated with age-related macular degeneration. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Boyan (Verified Customer) (08-27-2025) | good for WB, two bands at ~190 and 130 kd, both of which are C3 forms
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FH Marisa (Verified Customer) (07-31-2025) | We used the C3/C3b/C3c antibody in immunofluorescence and obtained a detectable signal, though some background was present. With slight optimization, it worked for visualizing complement activation by confocal microscopy.
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FH Marion (Verified Customer) (08-01-2024) | LPS induction model Fixative : Paraformaldehyde 4% Permeabilization: 10 min; RT; 0.2% Triton X-100 Blocking: 2 h; RT; 10% Normal donkey serum; 1% BSA Primary antibody: 1 night; 4°C Secondary antibody: ab150064 (Abcam); 1h30; RT Antibodies diluted in blocking solution
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