• Featured Product
  • KD/KO Validated

C3/C3b/C3c Polyklonaler Antikörper

C3/C3b/C3c Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (3)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(109)

Kat-Nr. : 21337-1-AP

Synonyme

C3, C3/C3a, Acylation stimulating protein, ARMD9, ASP



Geprüfte Anwendungen

Erfolgreiche Detektion in WBrat plasma, human plasma
Erfolgreiche IPhumanes Plasmagewebe
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Leberkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF-Phumanes Leberkarzinomgewebe
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)-PIF-P : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

21337-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA C3/C3b/C3c und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen C3/C3b/C3c fusion protein Ag15537
Vollständiger Name complement component 3
Berechnetes Molekulargewicht 1663 aa, 187 kDa
Beobachtetes Molekulargewicht 115 kDa
GenBank-ZugangsnummerBC150179
Gene symbol C3
Gene ID (NCBI) 718
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in activating the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3 (190-195 kDa), composed of α and β chains (115-120 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is composed of the α' chain and β chain (PMID: 27210597). Factor I cleaves the α′ chain of C3b to 68 kDa and 43 kDa degradation products (iC3b) (PMID: 25395424; 14527961). This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 α chain (115-120 kDa), C3b α' chain (110-115 kDa), and C3c α' chain fragment 2 (43 kDa).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for C3/C3b/C3c antibody 21337-1-APProtokoll herunterladen
IHC protocol for C3/C3b/C3c antibody 21337-1-APProtokoll herunterladenl
IF protocol for C3/C3b/C3c antibody 21337-1-APProtokoll herunterladen
IP protocol for C3/C3b/C3c antibody 21337-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

Autophagy enables microglia to engage amyloid plaques and prevents microglial senescence

Authors - Insup Choi
mouseWB

Brain Behav Immun

PDE4 inhibition alleviates HMGB1/C1q/C3-mediated excessive phagocytic pruning of synapses by microglia and depressive-like behaviors in mice

Authors - Qian Zhao

Brain

Extracellular vesicle biomarkers for complement dysfunction in schizophrenia

Authors - Ting Xue
IF

J Autoimmun

TIGIT-Fc fusion protein alleviates murine lupus nephritis through the regulation of SPI-B-PAX5-XBP1 axis-mediated B-cell differentiation

Authors - Junpeng Zhao
mouseIHC,IF

Gut Microbes

Targeted modulation of intestinal barrier and mucosal immune-related microbiota attenuates IgA nephropathy progression

Authors - Ran Zhang
humanIHC

Nat Commun

Increased circulating levels of Factor H-Related Protein 4 are strongly associated with age-related macular degeneration.

Authors - Valentina Cipriani

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (08-27-2025)

good for WB, two bands at ~190 and 130 kd, both of which are C3 forms

  • Applications: Western Blot
  • Cell Tissue Type: human
FH

Marisa (Verified Customer) (07-31-2025)

We used the C3/C3b/C3c antibody in immunofluorescence and obtained a detectable signal, though some background was present. With slight optimization, it worked for visualizing complement activation by confocal microscopy.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/100
  • Cell Tissue Type: Brain
C3/C3b/C3c Antibody Immunofluorescence validation (1/100 dilution) in Brain (Cat no:21337-1-AP)
FH

Marion (Verified Customer) (08-01-2024)

LPS induction model Fixative : Paraformaldehyde 4% Permeabilization: 10 min; RT; 0.2% Triton X-100 Blocking: 2 h; RT; 10% Normal donkey serum; 1% BSA Primary antibody: 1 night; 4°C Secondary antibody: ab150064 (Abcam); 1h30; RT Antibodies diluted in blocking solution

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Rat Kidney
C3/C3b/C3c Antibody Immunofluorescence validation (1/200 dilution) in Rat Kidney (Cat no:21337-1-AP)