C3/C3b/C3c Monoklonaler Antikörper

C3/C3b/C3c Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A6B3

Kat-Nr. : 66157-1-PBS

Synonyme

C3, C3/C3a, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, ASP, ARMD9

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  WB analysis using 66157-1-Ig (same clone as 66157-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66157-1-Ig (same clone as 66157-1-PBS)

  human plasma was subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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  WB analysis of human blood using 66157-1-Ig (same clone as 66157-1-PBS)

  human blood were subjected to SDS PAGE followed by western blot with 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66157-1-Ig (same clone as 66157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66157-1-Ig (same clone as 66157-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66157-1-Ig (C3/C3b/C3c antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66157-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66157-1-PBS bindet in WB, IHC, IF/ICC, ELISA C3/C3b/C3c und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C3/C3b/C3c fusion protein Ag15955
• Vollständiger Name: complement component 3
• Berechnetes Molekulargewicht: 1663 aa, 187 kDa
• Beobachtetes Molekulargewicht: 115 kDa
• GenBank-Zugangsnummer: BC150179
• Gene symbol: C3
• Gene ID (NCBI): 718
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The complement system is an important effector that bridges the innate and adaptive immune systems (PMID: 20010915). The third component of complement, C3, plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways (PMID: 11414361). Human C3, composed of α and β chains (115 and 75 kDa, respectively), is cleaved into C3a and C3b by C3 convertase. C3b is further cleaved into iC3b, C3c, C3dg and C3f. This antibody raised against 1314-1663 aa of human C3 protein recognizes the C3 alpha chain, C3b alpha' chain, and C3c alpha' chain fragment 2.