C3orf64 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C3orf64 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 27595-1-AP

Synonyme

EOGT, AER61, EC:2.4.1.255, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT1

---

• 

  WB analysis using 27595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27595-1-AP (C3orf64 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 27595-1-AP

  IP result of anti-C3orf64 (IP:27595-1-AP, 4ug; Detection:27595-1-AP 1:500) with mouse brain tissue lysate 4000 ug.

• 

  IHC staining of human breast cancer using 27595-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27595-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27595-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27595-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27595-1-AP (C3orf64 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

27595-1-AP bindet in WB, IHC, IF, IP, ELISA C3orf64 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C3orf64 fusion protein Ag25932
• Vollständiger Name: chromosome 3 open reading frame 64
• Beobachtetes Molekulargewicht: 60-62 kDa
• GenBank-Zugangsnummer: BC060887
• Gene symbol: C3orf64
• Gene ID (NCBI): 285203
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat,human	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	J Extracell Vesicles; Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease; Authors - Rong Yang
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	WB,IP,IF	Genomics; SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.; Authors - Can Yang ; KD Validated
human	WB	Proteomics; Solvent-induced proteome profiling for proteomic quantitation and target discovery of small molecular drugs; Authors - Chengli Yu
human	WB	Cartilage; HSF1 Increases EOGT-Mediated Glycosylation of Notch1 to Promote IL-1β-Induced Inflammatory Injury of Chondrocytes; Authors - Yuanchi Huang ; KD Validated