C4 Gamma Chain Monoklonaler Antikörper

C4 Gamma Chain Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G6A7

Kat-Nr. : 66242-1-PBS

Synonyme

C4 (gamma chain), C4A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4

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  WB analysis of human plasma using 66242-1-Ig (same clone as 66242-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66242-1-Ig (C4 gamma chain antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver using 66242-1-Ig (same clone as 66242-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using C4 Gamma Chain antibody (66242-1-Ig, Clone: 2G6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66242-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66242-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA C4 Gamma Chain und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C4 Gamma Chain fusion protein Ag10449
• Vollständiger Name: complement component 4A (Rodgers blood group)
• Berechnetes Molekulargewicht: 1744 aa, 193 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC012372
• Gene symbol: C4 Gamma Chain
• Gene ID (NCBI): 720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement C4, a component of the classical complement pathway, has an important role in innate immune function. C4 protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains (molecular wights 95, 78, and 31 kDa) prior to secretion. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. This antibody raised against 1543-1744aa of human C4-A protein recognizes C4 gamma chain.