ABHD18 Polyklonaler Antikörper

ABHD18 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25036-1-PBS

Synonyme

C4orf29, Abhydrolase domain-containing protein 18, Alpha/beta hydrolase domain-containing protein 18, Protein ABHD18

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  WB analysis of PC-3 using 25036-1-AP (same clone as 25036-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 25036-1-AP (same clone as 25036-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 25036-1-AP (same clone as 25036-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 25036-1-AP (C4orf29 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 25036-1-AP (same clone as 25036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 25036-1-AP (same clone as 25036-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25036-1-AP (C4orf29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 25036-1-AP (same clone as 25036-1-PBS)

  Immunofluorescent analysis of A549 cells using 25036-1-AP (C4orf29 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 25036-1-AP (same clone as 25036-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using C4orf29 antibody (25036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25036-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25036-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ABHD18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABHD18 fusion protein Ag19120
• Vollständiger Name: chromosome 4 open reading frame 29
• Berechnetes Molekulargewicht: 414 aa, 47 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC128143
• Gene symbol: C4orf29
• Gene ID (NCBI): 80167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABHD18 (α/β hydrolase domain-containing protein 18) is a transmembrane protein in mammals whose function has not yet been fully elucidated. It belongs to the large α/β hydrolase superfamily, whose members typically possess hydrolase activity and are involved in a variety of metabolic reactions. The ABHD18 protein is localized on the lysosomal membrane and may participate in intracellular lipid metabolism or signal transduction processes. Recent studies have shown that ABHD18 is the key enzyme in human cells that catalyzes the deacylation of cardiolipin to MLCL. The loss of ABHD18 function can significantly alleviate mitochondrial supercomplex defects, energy metabolism disorders, and cardiomyopathy phenotypes caused by TAZ deficiency, even achieving a "genetic suppression" effect in mice and patient cells. This not only reveals ABHD18 as a core node in the cardiolipin remodeling pathway but also provides an entirely new therapeutic approach for mitochondrial diseases such as Barth syndrome.