C5 Polyklonaler Antikörper

C5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22492-1-AP

Synonyme

C5, C5b, C5, C5a, Complement C5 alpha chain, Complement C5, C5a anaphylatoxin

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  WB analysis using 22492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22492-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 22492-1-AP (C5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, rat plasma, mouse serum

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22492-1-AP bindet in WB, ELISA C5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C5 fusion protein Ag17882
• Vollständiger Name: complement component 5
• Berechnetes Molekulargewicht: 1676 aa, 188 kDa
• Beobachtetes Molekulargewicht: 120 kDa, 70 kDa
• GenBank-Zugangsnummer: BC113740
• Gene symbol: C5
• Gene ID (NCBI): 727
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)