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C5a Polyklonaler Antikörper

C5a Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32767-1-AP

Synonyme

C5, C5a anaphylatoxin, Complement C5, Complement C5 alpha chain, Complement C5 alpha' chain

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  • Western Blot (WB) analysis of various lysates using C5a Polyclonal antibody (32767-1-AP)

    WB analysis using 32767-1-AP

    Mouse serum samples was subjected to SDS PAGE, followed by western blot with 32767-1-AP (Component C5a antibody) at a dilution of 1:1000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBmouse serum

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

32767-1-AP bindet in WB, ELISA C5a und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen C5a fusion protein Eg3332
Vollständiger Name hemolytic complement
Berechnetes Molekulargewicht189kDa
Beobachtetes Molekulargewicht110 kDa
GenBank-ZugangsnummerNM_010406.2
Gene symbol Hc
Gene ID (NCBI) 15139
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for C5a antibody 32767-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
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