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C5AR1/CD88 Rekombinanter Antikörper

C5AR1/CD88 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241712G8

Kat-Nr. : 84336-6-PBS

Synonyme

C5AR, C5AR1, 241712G8, C5A, C5a anaphylatoxin chemotactic receptor 1

Filter:
  • Western Blot (WB) analysis of various lysates using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    WB analysis using 84336-6-RR (same clone as 84336-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84336-6-RR (C5AR1/CD88 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84336-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using C5AR1/CD88 Recombinant antibody (84336-6-RR)

    IF Staining of THP-1 using 84336-6-RR (same clone as 84336-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using C5AR1/CD88 antibody (84336-6-RR, Clone: 241712G8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84336-6-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84336-6-PBS bindet in WB, IF/ICC, Indirect ELISA C5AR1/CD88 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen C5AR1/CD88 fusion protein Eg2185
Vollständiger Name complement component 5a receptor 1
Berechnetes Molekulargewicht39kDa
Beobachtetes Molekulargewicht45 kDa
GenBank-ZugangsnummerNM_001736.4
Gene symbol C5aR
Gene ID (NCBI) 728
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. The deduced sequence of C5aR contains 350 amino acids, giving a calculated molecular weight of 39 kDa. C5aR has an N-linked glycosylation site in the N-terminal extracellular domain. The apparent molecular weight of C5aR is about 40-52 kDa (PMID: 1847994; 9136907; 9136907; 2007135).