C5AR1/CD88 Rekombinanter Antikörper

C5AR1/CD88 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241712D4

Kat-Nr. : 98226-1-PBS

Synonyme

C5AR, C5AR1, 241712D4, C5A, C5a anaphylatoxin chemotactic receptor 1

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  FC experiment of human peripheral blood leukocytes using 98226-1-RR (same clone as 98226-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98226-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human peripheral blood leukocytes using 98226-1-RR (same clone as 98226-1-PBS)

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human C5AR1/CD88 Rabbit Recombinant Antibody (98226-1-RR, Clone: 241712D4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98226-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98226-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98226-1-RR against Human C5AR1/CD88 were performed. The affinity constant is 1.56 nM.

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Geprüfte Anwendungen

Produktinformation

98226-1-PBS bindet in FC C5AR1/CD88 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: C5AR1/CD88 fusion protein Eg2185
• Vollständiger Name: complement component 5a receptor 1
• Berechnetes Molekulargewicht: 39kDa
• GenBank-Zugangsnummer: NM_001736.4
• Gene symbol: C5aR
• Gene ID (NCBI): 728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

C5aR, also named CD88, is a G protein-coupled receptor for the anaphylatoxin C5a, a cleavage product of the complement cascade. The C5a ligand is a proinflammatory component of host defense. C5aR is activated upon binding of the C5a molecule. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production.