C5orf24 Polyklonaler Antikörper

C5orf24 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24319-1-AP

Synonyme

chromosome 5 open reading frame 24

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  WB analysis of HEK-293 using 24319-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24319-1-AP (C5orf24 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24319-1-AP

  IP result of anti-C5orf24 (IP:24319-1-AP, 4ug; Detection:24319-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

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  IHC staining of human pancreas using 24319-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 24319-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24319-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 24319-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24319-1-AP (C5orf24 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 24319-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C5orf24 antibody (24319-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 24319-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug C5orf24 Polyclonal antibody (24319-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24319-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA C5orf24 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C5orf24 fusion protein Ag19534
• Vollständiger Name: chromosome 5 open reading frame 24
• Berechnetes Molekulargewicht: 188 aa, 20 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC053677
• Gene symbol: C5orf24
• Gene ID (NCBI): 134553
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C5orf24 is a cellular transcriptional regulator that performs different functions in different cell types, including the processes of cell proliferation, cell polarization, migration, and tumor development.C5orf24 is able to specifically and efficiently capture a wide range of tuberculosis antigens, which is important for the diagnosis of tuberculosis. C5orf24 has two isoforms with MW 20 kDa and 16 kDa. For phosphoserine modification, the MW is about 20-23 kDa.

Publikationen

Species	Application	Title
pig	WB	Front Immunol; Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.; Authors - Xu Ye