C5orf4 Polyklonaler Antikörper

C5orf4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 22046-1-AP

Synonyme

C5orf4, fatty acid hydroxylase domain containing 2, FAXDC2, Uncharacterized protein C5orf4

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  WB analysis of COLO 320 using 22046-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22046-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22046-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22046-1-AP (C5orf4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22046-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22046-1-AP (C5orf4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, A549-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

22046-1-AP bindet in WB, IHC, ELISA C5orf4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C5orf4 fusion protein Ag16960
• Vollständiger Name: chromosome 5 open reading frame 4
• Berechnetes Molekulargewicht: 333 aa, 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC007216
• Gene symbol: C5orf4
• Gene ID (NCBI): 10826
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid hydroxylase domain containing 2 (FAXDC2), also known as C5orf4, is a 40 kDa protein. The function of FAXDC2 remains largely unknown. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.

Publikationen

Species	Application	Title
human	WB	Blood Cancer J; Novel function of FAXDC2 in megakaryopoiesis.; Authors - Q Jin
mouse	WB	J Clin Invest; The cholesterol biosynthesis enzyme FAXDC2 couples Wnt/β-catenin to RTK/MAPK signaling; Authors - Babita Madan