ATAT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ATAT1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 28828-1-AP

Synonyme

C6orf134, Nbla00487, MEC17, FLJ13158, alpha-TAT1

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  WB analysis using 28828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28828-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 28828-1-AP (ATAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (ATAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 28828-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28828-1-AP (C6orf134 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 28828-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATAT1 antibody (28828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of A431 using 28828-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human ATAT1 (28828-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, THP-1-Zellen, U-251-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: Rattenhodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below

Produktinformation

28828-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA ATAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ATAT1 fusion protein Ag28757
• Vollständiger Name: chromosome 6 open reading frame 134
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: NM_001031722
• Gene symbol: ATAT1
• Gene ID (NCBI): 79969
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C6orf134 is a reported alias name for the human gene ATAT1 coding alpha-tubulin N-acetyltransferase 1, and specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. May affect microtubule stability and regulate microtubule dynamics. May be involved in neuron development. Western blot shows a band between 36 kDa and 45 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	iScience; MFN2 coordinates mitochondria motility with α-tubulin acetylation and this regulation is disrupted in CMT2A; Authors - Atul Kumar
rat	WB	Biochem Biophys Res Commun; NWD1 influences the extension of neuronal axons by regulating microtubule stability; Authors - Tiancheng Bao
human	WB	Cancer Cell Int; Suppression of microtubule acetylation mediates the anti-leukemic effect of CDK9 inhibition; Authors - Xi Xie ; KD Validated
mouse	WB	Cell Death Dis; TMEM232 is required for the formation of sperm flagellum and male fertility in mice; Authors - Xinying Cai
	IF	Clin Transl Med; Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition; Authors - Hui Luo
		Sci China Life Sci; FSD1 inhibits glioblastoma diffuse infiltration through restriction of HDAC6-mediated microtubule deacetylation; Authors - Dake Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

K. (Verified Customer) (10-26-2023)

Each well had 20 ug of protein extract added. On a Nitrocellulose Blotting Membrane, transfer was carried out for two hours at 400 mA at 4 oC. Membrane blocking was carried out at room temperature with 5% BSA in TBS-Tween20 under light shaking. Antibodies were diluted in PBS-Tween20 containing 5% non-fat milk. -The primary antibody incubations were carried out as follows: 1:1000 for three hours at room temperature.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human and mouse Liver cells