• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

C7orf47 Polyklonaler Antikörper

C7orf47 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24214-1-AP

Synonyme

C7orf47, UPF0683 protein C7orf47

Galerie der Validierungsdaten

Filter:
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C7orf47 Polyclonal antibody (24214-1-AP)

    IHC staining of human breast cancer using 24214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C7orf47 Polyclonal antibody (24214-1-AP)

    IHC staining of human breast cancer using 24214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using C7orf47 Polyclonal antibody (24214-1-AP)

    IHC staining of human breast cancer using 24214-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C7orf47 Polyclonal antibody (24214-1-AP)

    IHC staining of human testis using 24214-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using C7orf47 Polyclonal antibody (24214-1-AP)

    IHC staining of human testis using 24214-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24214-1-AP (C7orf47 Antibody) at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IFSee 1 publications below

Produktinformation

24214-1-AP bindet in IHC, IF, ELISA C7orf47 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen C7orf47 fusion protein Ag18340
Vollständiger Name chromosome 7 open reading frame 47
Berechnetes Molekulargewicht 253 aa, 28 kDa
GenBank-ZugangsnummerBC026269
Gene symbol C7orf47
Gene ID (NCBI) 221908
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-affinitätsgereinigt
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

Produktspezifische Protokolle
IHC protocol for C7orf47 antibody 24214-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Elife

PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.

Authors - Andrew Michael Sydor
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Elisa (Verified Customer) (04-24-2023)

Weak centriolar staining. While this antibody works well for Westernblotting, the expected centriolar staining in IF is rather weak. Yet, this antibody works under the following conditions: RPE1 cells stained for Hoechst (DNA marker, in green), PPP1r35 (centriolar marker, in magenta) and g-Tubulin (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:300 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)
C7orf47 Antibody Immunofluorescence validation (1:50 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:24214-1-AP)