C8G Polyklonaler Antikörper

C8G Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27200-1-AP

Synonyme

C8C

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  WB analysis using 27200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27200-1-AP (C8G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 27200-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 27200-1-AP (C8G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27200-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27200-1-AP (C8G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human milk, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27200-1-AP bindet in WB, IHC, ELISA C8G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C8G fusion protein Ag17949
• Vollständiger Name: complement component 8, gamma polypeptide
• Berechnetes Molekulargewicht: 202 aa, 22 kDa
• Beobachtetes Molekulargewicht: 18-22 kDa
• GenBank-Zugangsnummer: BC113626
• Gene symbol: C8G
• Gene ID (NCBI): 733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gamma chain of complement component 8 (C8G) is one of three different subunits (alpha, beta, and gamma) of the eighth complement component (C8), which is a component of the MAC (membrane attack complex) (PMID:33382892). Although the roles of C8A and C8B are directly associated with MAC formation, C8G is not essential in the assembly and activity of the MAC (PMID:11960635). Moreover, C8G was shown to inhibit neuroinflammation by interfering with the sphingosine-1-phosphate (S1P)-S1PR2 interaction (PMID:34149451).