AFAF/EQTN Polyklonaler Antikörper

AFAF/EQTN Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23168-1-AP

Synonyme

AFAF, C9orf11, EQTN, Equatorin

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  WB analysis of HepG2 using 23168-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23168-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23168-1-AP (AFAF/EQTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 23168-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFAF/EQTN antibody (23168-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23168-1-AP bindet in WB, IF/ICC, ELISA AFAF/EQTN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AFAF/EQTN fusion protein Ag19656
• Vollständiger Name: chromosome 9 open reading frame 11
• Berechnetes Molekulargewicht: 294 aa, 33 kDa
• Beobachtetes Molekulargewicht: 27-29 kDa
• GenBank-Zugangsnummer: BC137351
• Gene symbol: AFAF
• Gene ID (NCBI): 54586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The AFAF gene (also known as EQTN and C9orf11) encodes a membrane protein that is specifically localized to the acrosomal membrane. AFAF is involved in the process of fertilization and acrosome biogenesis. Expression analysis demonstrates that AFAF is highly expressed in the testis, although minor expression was seen in other tissues. AFAF has 3 isoforms with molecular weights of 14, 29, and 33 kDa. However, one article detected molecular weight between 40-65 kDa in mouse testis (PMID: 19605790).