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C9orf117 Polyklonaler Antikörper

C9orf117 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21012-1-PBS

Synonyme

AFAF, Cilia- and flagella-associated protein 157, EQTN, Equatorin

Filter:
  • Western Blot (WB) analysis of various lysates using C9orf117 Polyclonal antibody (21012-1-AP)

    WB analysis using 21012-1-AP (same clone as 21012-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21012-1-AP (C9orf117 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using C9orf117 Polyclonal antibody (21012-1-AP)

    IHC staining of mouse brain using 21012-1-AP (same clone as 21012-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21012-1-AP (C9orf117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using C9orf117 Polyclonal antibody (21012-1-AP)

    IF Staining of A549 using 21012-1-AP (same clone as 21012-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using C9orf117 antibody (21012-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21012-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

21012-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA C9orf117 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen C9orf117 fusion protein Ag15234
Vollständiger Name chromosome 9 open reading frame 117
Berechnetes Molekulargewicht 520 aa, 61 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerBC141809
Gene symbol C9orf117
Gene ID (NCBI) 286207
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C9orf117 (Cilia- and flagella-associated protein 157, CFAP157) protein localizes to basal bodies and interacts with tubulin and the centrosomal protein CEP350 (PMID: 27965440).