C9orf41/CARNMT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C9orf41/CARNMT1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 24068-1-AP

Synonyme

C9orf41, CARNMT1, Carnosine N-methyltransferase, EC:2.1.1.22

---

• 

  WB analysis of HepG2 using 24068-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24068-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24068-1-AP (C9orf41/CARNMT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 24068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf41/CARNMT1 antibody (24068-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

24068-1-AP bindet in WB, IF/ICC, ELISA C9orf41/CARNMT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C9orf41/CARNMT1 fusion protein Ag21268
• Vollständiger Name: chromosome 9 open reading frame 41
• Berechnetes Molekulargewicht: 409 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC034033
• Gene symbol: C9orf41
• Gene ID (NCBI): 138199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The enzyme CARNMT1 (carnosine N-methyltransferase 1, also known as C9orf41), conserved from yeast to human, was identified as an N1 position-specific methyltransferase that catalyzes the conversion of carnosine to anserine (β-alanyl-N-π-methyl-L-histidine).

Publikationen

Species	Application	Title
mouse	WB	Genes Dev; Histidine N1-position-specific methyltransferase CARNMT1 targets C3H zinc finger proteins and modulates RNA metabolism; Authors - Tadahiro Shimazu ; KO Validated