C9orf61 Polyklonaler Antikörper

C9orf61 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 25630-1-AP

Synonyme

Endosomal transmembrane binding with epsin, Endosomal transmembrane epsin interactor 1, ENTREP1, FAM189A2, Protein FAM189A2

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  WB analysis of mouse skeletal muscle using 25630-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 25630-1-AP (C9orf61 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 25630-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25630-1-AP (C9orf61 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

25630-1-AP bindet in WB, IHC, CoIP, ELISA C9orf61 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C9orf61 fusion protein Ag22242
• Vollständiger Name: chromosome 9 open reading frame 61
• Berechnetes Molekulargewicht: 450 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC113685
• Gene symbol: C9orf61
• Gene ID (NCBI): 9413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.

Publikationen

Species	Application	Title
human	WB,IHC,CoIP	Tissue Cell; FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins; Authors - Jiakun Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chrisovalantou (Verified Customer) (10-31-2024)

Antibody unfortunately could not reliably and specifically detect the protein of interest. Many unspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y, HEK293T

C9orf61 Antibody Western Blot validation (1:1000 dilution) in SH-SY5Y, HEK293T (Cat no:25630-1-AP)