C9orf78 Polyklonaler Antikörper

C9orf78 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14496-1-PBS

Synonyme

TLS1, HSPC220, Hepatocellular carcinoma-associated antigen 59, HCA59, CSU2

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  WB analysis using 14496-1-AP (same clone as 14496-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14496-1-AP (C9orf78 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

  IP result of anti-C9orf78 (IP:14496-1-AP, 4ug; Detection:14496-1-AP 1:500) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 14496-1-AP (same clone as 14496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14496-1-AP (C9orf78 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 14496-1-AP (same clone as 14496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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  IHC staining of human bladder using 14496-1-AP (same clone as 14496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bladder using 14496-1-AP (C9orf78 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 14496-1-AP (C9orf78 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14496-1-AP (same clone as 14496-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using C9orf78 antibody (14496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 14496-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14496-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA C9orf78 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C9orf78 fusion protein Ag5919
• Vollständiger Name: chromosome 9 open reading frame 78
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC007664
• Gene symbol: C9orf78
• Gene ID (NCBI): 51759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The function of C9orf78 remains largely unknown. The MW of this protein is 34 kDa, and this antibody specially recognises the 34 kDa protein. This antibody works well in WB, IHC and IF applications.