C9orf89 Rekombinanter Antikörper

C9orf89 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242638F7

Kat-Nr. : 85357-1-PBS

Synonyme

242638F7, Bcl10-interacting CARD protein, BinCARD, CARD19, Caspase recruitment domain-containing protein 19

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  WB analysis using 85357-1-RR (same clone as 85357-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85357-1-RR (C9orf89 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85357-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85357-1-RR (same clone as 85357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85357-1-RR (C9orf89 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85357-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85357-1-RR (same clone as 85357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85357-1-RR (C9orf89 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85357-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 85357-1-RR (same clone as 85357-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using C9orf89 antibody (85357-1-RR, Clone: 242638F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85357-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85357-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85357-1-RR against Human C9orf89 were performed. The affinity constant is 0.756 nM.

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Geprüfte Anwendungen

Produktinformation

85357-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA C9orf89 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: C9orf89 fusion protein Ag14084
• Vollständiger Name: chromosome 9 open reading frame 89
• Berechnetes Molekulargewicht: 228 aa, 26 kDa
• Beobachtetes Molekulargewicht: 15-21 kDa
• GenBank-Zugangsnummer: BC004500
• Gene symbol: C9orf89
• Gene ID (NCBI): 84270
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C9orf89 (also known as CARD19 or BinCARD) is a mitochondria-localized protein that inhibits BCL10-induced NF-κB activation mainly through its caspase recruitment structural domain (CARD). It plays a role in T cell receptor (TCR) signaling, but deletion of endogenous CARD19 has a lesser effect on Bcl10-dependent NF-κB activation. In addition, C9orf89 is expressed in multiple cell types and is involved in the regulation of inflammatory and immune responses.