SPACA9 Polyklonaler Antikörper

SPACA9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26034-1-AP

Synonyme

C9orf9, Sperm acrosome-associated protein 9, Uncharacterized protein C9orf9

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  WB analysis of mouse testis using 26034-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 26034-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 26034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 26034-1-AP (C9orf9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 26034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26034-1-AP (SPACA9 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 26034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26034-1-AP (SPACA9 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 26034-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SPACA9 antibody (26034-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat testis using 26034-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat testis tissue using SPACA9 antibody (26034-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 26034-1-AP

  Immunofluorescent analysis of A431 cells using 26034-1-AP (C9orf9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HaCaT using 26034-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using SPACA9 antibody (26034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 26034-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPACA9 antibody (26034-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Mauslebergewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen, HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26034-1-AP bindet in WB, IHC, IF/ICC, ELISA SPACA9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPACA9 fusion protein Ag23320
• Vollständiger Name: chromosome 9 open reading frame 9
• Beobachtetes Molekulargewicht: 18 kDa, 30 kDa
• GenBank-Zugangsnummer: BC012940
• Gene symbol: SPACA9
• Gene ID (NCBI): 11092
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The function of C9orf9 remains largely unknown. Catalog#26034-1-AP is a rabbit polyclonal antibody raised against the full-length of human C9orf9. Two isoforms were produced by alternative splicing with predicted MW of 19 and 26 kDa (UniProt). Two bands at 16 kDa and 30 kDa could be detected using the present rabbit polyclonal antibody 26034-1-AP.

Publikationen

Species	Application	Title
mouse	WB	Curr Biol; CCDC176 stabilizes microtubule doublets 1 and 9 to ensure proper sperm movement; Authors - Chao Liu
mouse	WB	Cell Mol Life Sci; CCDC146 is required for sperm flagellum biogenesis and male fertility in mice; Authors - Yanjie Ma