CA1 Polyklonaler Antikörper

CA1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 13198-2-AP

Synonyme

CA I, CA1, CAB, Car1, Carbonate dehydratase I, Carbonic anhydrase 1, Carbonic anhydrase B, carbonic anhydrase I

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Galerie der Validierungsdaten

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  WB analysis using 13198-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 13198-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 13198-2-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13198-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13198-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13198-2-AP (CA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 13198-2-AP

  IP Result of anti-CA1 (IP:13198-2-AP, 4ug; Detection:13198-2-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human breast cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13198-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13198-2-AP (CA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Jurkat-Zellen, Rattenmilzgewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

13198-2-AP bindet in WB, IP, IHC, IF, ELISA CA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA1 fusion protein Ag3932
• Vollständiger Name: carbonic anhydrase I
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC027890
• Gene symbol: CA1
• Gene ID (NCBI): 759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA1(Carbonic anhydrase 1) is also named as CAB and belongs to the alpha-carbonic anhydrase family, which may reside in cytoplasm, in mitochondria, or in secretory granules, or associate with membranes in cell. It forms a large family of genes encoding zinc metalloenzymes of great physiologic importance. Extracellular CA1 mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation(PMID:17259996).

Publikationen

Species	Application	Title
mouse,rat	WB,IF	Acta Neuropathol Commun; Upregulation of carbonic anhydrase 1 beneficial for depressive disorder; Authors - Ke Cheng ; KO Validated
rat	WB	J Ginseng Res; Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway; Authors - Mei-Li Lu
mouse	IF	FASEB J; Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.; Authors - Brittany R Jenkins
human	WB	J Inflamm Res; M1-Type Macrophages Secrete TNF-α to Stimulate Vascular Calcification by Upregulating CA1 and CA2 Expression in VSMCs; Authors - Xianqin Song
mouse	WB	Am J Physiol Lung Cell Mol Physiol; Carbonic anhydrase and soluble adenylate cyclase regulation of cystic fibrosis cellular phenotypes.; Authors - Kathleen Boyne
human,rat	WB	Front Pharmacol; Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.; Authors - Meili Lu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brittany (Verified Customer) (09-27-2019)

Using the CA-1 primary antibody with mouse colon scrapes yielded very clean bands with western blotting. Immunofluorescence also gave a fairly clean, detectable signal, with CA-1 showing up along the brush border in mouse colon tissue. There were some cells that stained more intracellularly, and unsure if that was non-specific binding of the antibody, or another cell type other than colonocytes that expresses CA-1 intracellularly.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (WB) and 1:100 (IF)
• Cell Tissue Type: Colon tissue (scrapes for western blot)

CA1 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB) and 1:100 (IF) dilution) in Colon tissue (scrapes for western blot) (Cat no:13198-2-AP)