CA11 Polyklonaler Antikörper

CA11 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15435-1-AP

Synonyme

CA RP II, CA RP XI, CA XI, CA11, carbonic anhydrase XI, CARP 2, CARP XI, CARP2, CARPX1

---

Galerie der Validierungsdaten

• 

  WB analysis of human kidney using 15435-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 15435-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 15435-1-AP (CA11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15435-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15435-1-AP (CA11 antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15435-1-AP bindet in WB, IHC, ELISA CA11 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA11 fusion protein Ag7712
• Vollständiger Name: carbonic anhydrase XI
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kda, 45 kDa
• GenBank-Zugangsnummer: BC002662
• Gene symbol: CA11
• Gene ID (NCBI): 770
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA11(Carbonic anhydrase-related protein 11) is also named as CARP2, CARP XI and belongs to the alpha-carbonic anhydrase family. CARP XI is observed in the cerebellum, cerebrum, liver, stomach, small intestine, colon, kidney, and testis by immunohistochemical and in the cerebellum, the most prominent signal is located in the Purkinje cells(PMID:20356370). CARP XI contains a hydrophobic N-terminal region for a possible signal sequence and asparagine glycosylation site and it plays a general role in the human central nervous system(PMID: 10350627).