CA12 Monoklonaler Antikörper

CA12 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E1D8

Kat-Nr. : 68224-1-PBS

Synonyme

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

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  WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

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  WB analysis using 68224-1-Ig (same clone as 68224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68224-1-Ig (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 68224-1-Ig (same clone as 68224-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CA12 antibody (68224-1-Ig, Clone: 3E1D8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68224-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68224-1-PBS bindet in WB, IF/ICC, Indirect ELISA CA12 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CA12 fusion protein Ag7235
• Vollständiger Name: carbonic anhydrase XII
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC000278
• Gene symbol: CA12
• Gene ID (NCBI): 771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.