CA12 Rekombinanter Antikörper

CA12 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

243053B2

Kat-Nr. : 85595-3-PBS

Synonyme

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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  WB analysis using 85595-3-RR (same clone as 85595-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85595-3-RR (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85595-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01993-1

  Sandwich ELISA standard curve of MP01993-1, Human CA12 Recombinant Matched Antibody Pair - PBS only. 85595-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7107. 85595-2-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

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  Sample test of MP01993-1

  The mean CA12 concentration was determined to be 93.99 ng/mL in A549 cell extract based on a 3.10 mg/mL extract load.

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  Cytometric bead array standard curve of MP01993-1

  Cytometric bead array standard curve of MP01993-1, CA12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85595-3-PBS. Detection antibody: 85595-2-PBS. Standard: Ag7107. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85595-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85595-3-RR against Human CA12 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85595-3-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CA12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CA12 fusion protein Ag7107
• Vollständiger Name: carbonic anhydrase XII
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC000278
• Gene symbol: CA12
• Gene ID (NCBI): 771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.