CA12 Rekombinanter Antikörper

CA12 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243053B2

Kat-Nr. : 85595-3-RR

Synonyme

CA XII, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII

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  WB analysis using 85595-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85595-3-RR (CA12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85595-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85595-3-RR against Human CA12 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mausnierengewebe, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85595-3-RR bindet in WB, ELISA CA12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CA12 fusion protein Ag7107
• Vollständiger Name: carbonic anhydrase XII
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC000278
• Gene symbol: CA12
• Gene ID (NCBI): 771
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA12 belongs to the alpha-carbonic anhydrase family. It reverses the hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.