CA13 Rekombinanter Antikörper

CA13 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250787A12

Kat-Nr. : 86178-1-RR

Synonyme

CA XIII, Carbonate dehydratase XIII, Carbonic anhydrase 13, Carbonic anhydrase XIII, CAXIII

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  WB analysis of HepG2 using 86178-1-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86178-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 86178-1-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86178-1-RR (CA13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86178-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86178-1-RR against Human CA13 were performed. The affinity constant is 4.50 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maus-Thymusgewebe, NIH/3T3-Zellen, Ratten-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86178-1-RR bindet in WB, ELISA CA13 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CA13 fusion protein Ag10086
• Vollständiger Name: carbonic anhydrase XIII
• Berechnetes Molekulargewicht: 262 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC052602
• Gene symbol: CA13
• Gene ID (NCBI): 377677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carbonic anhydrases (CAs) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide. The mammalian α-CA gene family has been reported to include at least eleven enzymatically active isoforms with different structural and catalytic properties. Four of the active CA isozymes are cytosolic (CA I, II, III, and VII), four are membrane-associated(CA IV, IX, XII, and XIV), two are mitochondrial (CA VA and VB), and one is a secretory form (CA VI). CA13 is a cytosolic protein that may play a role in embryogenesis and perturbation of its function by genetic modification could potentially lead to developmental abnormalities(PMID:14600151).