MUC1/CA15-3 C-terminal Rekombinanter Antikörper

MUC1/CA15-3 C-terminal Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240190G10

Kat-Nr. : 83311-4-PBS

Synonyme

CA15-3,MUC1, CA15-3, CA153, MUC1, CA15 3

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  WB analysis using 83311-4-RR (same clone as 83311-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83311-4-RR (MUC1/CA15-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83311-4-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 83311-4-RR (same clone as 83311-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83311-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 83311-4-RR (same clone as 83311-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83311-4-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 83311-4-RR (same clone as 83311-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83311-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83311-4-RR (same clone as 83311-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83311-4-RR (MUC1/CA15-3 C-terminal antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83311-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83311-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83311-4-RR against Human CA15-3/MUC1 were performed. The affinity constant is 63.7 pM.

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Geprüfte Anwendungen

Produktinformation

83311-4-PBS bindet in WB, IHC, ELISA MUC1/CA15-3 C-terminal und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: MUC1/CA15-3 C-terminal fusion protein Ag20366
• Vollständiger Name: mucin 1, cell surface associated
• Berechnetes Molekulargewicht: 1264 aa, 123 kDa
• Beobachtetes Molekulargewicht: 23-30 kDa
• GenBank-Zugangsnummer: BC120975
• Gene symbol: MUC1/CA15-3
• Gene ID (NCBI): 4582
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of the female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.