CA2 Polyklonaler Antikörper

CA2 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16961-1-PBS

Synonyme

Carbonate dehydratase II, Car2, CA-II, CAII, CAC

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  WB analysis using 16961-1-AP (same clone as 16961-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of rat kidney using 16961-1-AP (same clone as 16961-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of human stomach using 16961-1-AP (same clone as 16961-1-PBS)

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 16961-1-AP (same clone as 16961-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse colon using 16961-1-AP (same clone as 16961-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 16961-1-AP (same clone as 16961-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 16961-1-AP (CA2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 16961-1-AP (same clone as 16961-1-PBS)

  IP result of anti-CA2 (IP:16961-1-AP, 4ug; Detection:16961-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16961-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16961-1-PBS bindet in WB, IP, Indirect ELISA CA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CA2 fusion protein Ag10680
• Vollständiger Name: carbonic anhydrase II
• Berechnetes Molekulargewicht: 260 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC011949
• Gene symbol: CA2
• Gene ID (NCBI): 760
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA2(carbonic anhydrase II ) is also called Car2, CA-II, and CAII and belongs to the alpha-carbonic anhydrase family. It reversibly hydrates CO2 in cellular ion transport and homeostasis. CA2 is essential for bone resorption and osteoclast differentiation. It regulates SLC9A1 activity via a phosphorylation-regulated CAII binding site in SLC9A1 tail(PMID:16475831). It also may be involved in brain development (PMID:16825953). Defects in CA2 cause osteopetrosis autosomal recessive type 3 (OPTB3).