CA3 Monoklonaler Antikörper

CA3 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C10A2

Kat-Nr. : 66608-1-PBS

Synonyme

Carbonic anhydrase III, 3C10A2, CA 3, CA III, CAIII

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  WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66608-1-Ig (same clone as 66608-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66608-1-Ig (CA3 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66608-1-Ig (CA3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse skeletal muscle using 66608-1-Ig (same clone as 66608-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using CA3 antibody (66608-1-Ig, Clone: 3C10A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66608-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66608-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CA3 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CA3 fusion protein Ag7513
• Vollständiger Name: carbonic anhydrase III, muscle specific
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC004897
• Gene symbol: CA3
• Gene ID (NCBI): 761
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).