Carbonic Anhydrase 4/CA4 Polyklonaler Antikörper

Carbonic Anhydrase 4/CA4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 13931-1-AP

Synonyme

CA4, CA IV, CAIV, CA-IV, Car4

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  WB analysis using 13931-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13931-1-AP (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13931-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13931-1-AP (Carbonic Anhydrase 4/CA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 13931-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 13931-1-AP (Carbonic Anhydrase 4/CA4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 13931-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13931-1-AP (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13931-1-AP

  Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 13931-1-AP (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Rattenlungengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

13931-1-AP bindet in WB, IHC, IF, ELISA Carbonic Anhydrase 4/CA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carbonic Anhydrase 4/CA4 fusion protein Ag4960
• Vollständiger Name: carbonic anhydrase IV
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC057792
• Gene symbol: CA4
• Gene ID (NCBI): 762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressing on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.

Publikationen

Species	Application	Title
human	IHC	Theranostics; Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype; Authors - Zhi-Jie Gao
	WB	Sci Rep; Carbonic anhydrase IV in lizard chemical signals; Authors - Marco Mangiacotti
human	WB	J Cancer; Down-regulated C4orf19 confers poor prognosis in colon adenocarcinoma identified by gene co-expression network.; Authors - Wanpeng Wang
human	IHC	Nat Commun; A CCL2+DPP4+ subset of mesenchymal stem cells expedites aberrant formation of creeping fat in humans; Authors - Fengfei Wu
human	IF	Am J Respir Cell Mol Biol; Activated Clec4nhi Neutrophils Aggravate Lung Injury in an Endothelial IGFBP7 Dependent Manner; Authors - Linchao Yu