Carbonic Anhydrase 4/CA4 Rekombinanter Antikörper

Carbonic Anhydrase 4/CA4 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242975A3

Kat-Nr. : 85706-1-PBS

Synonyme

CA4, CA IV, CAIV, CA-IV, Car4

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  WB analysis using 85706-1-RR (same clone as 85706-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85706-1-RR (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85706-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 85706-1-RR (same clone as 85706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 85706-1-RR (Carbonic Anhydrase 4/CA4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85706-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85706-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85706-1-RR against Human CA4 were performed. The affinity constant is 9.62 nM.

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Geprüfte Anwendungen

Produktinformation

85706-1-PBS bindet in WB, IHC, Indirect ELISA Carbonic Anhydrase 4/CA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Carbonic Anhydrase 4/CA4 fusion protein Ag4960
• Vollständiger Name: carbonic anhydrase IV
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC057792
• Gene symbol: CA4
• Gene ID (NCBI): 762
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA4(Carbonic anhydrase 4) is a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary, chorionic (and certain other) capillaries and of proximal renal tubules. It plays a critical role by maintaining the pH in the outer retina, which is important for the normal function of photoreceptors. This protein belongs to the alpha-carbonic anhydrase family.