Carbonic Anhydrase IX/CA9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Carbonic Anhydrase IX/CA9 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(49)

Rezensionen (5)

Kat-Nr. : 11071-1-AP

Synonyme

CA9, CAIX, carbonic anhydrase IX, CA 9, CA IX

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• 

  WB analysis of A549 using 11071-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11071-1-AP

  Non-treated and Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11071-1-AP

  WB result of Carbonic Anhydrase IX/CA9 antibody (11071-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Carbonic Anhydrase IX/CA9 transfected A549 cells.

• 

  IP experiment of HeLa using 11071-1-AP

  IP result of anti-Carbonic Anhydrase IX/CA9 (IP:11071-1-AP, 4ug; Detection:11071-1-AP 1:3000) with HeLa cells lysate 1200 ug.

• 

  IHC staining of human stomach cancer using 11071-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11071-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11071-1-AP (Carbonic Anhydrase IX/CA9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, mit Cobaltchlorid behandelte HeLa-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IHC: See 27 publications below
• IF: See 9 publications below

Produktinformation

11071-1-AP bindet in WB, IHC, IF, IP, ELISA Carbonic Anhydrase IX/CA9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carbonic Anhydrase IX/CA9 fusion protein Ag1540
• Vollständiger Name: carbonic anhydrase IX
• Berechnetes Molekulargewicht: 459 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 60-70 kDa
• GenBank-Zugangsnummer: BC014950
• Gene symbol: CA9
• Gene ID (NCBI): 768
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CA9 (Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia (PMID:18703501). It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. The protein is present in the cytoplasmic membrane and the nucleus, with a molecular weight of 50 kDa. The molecular weight of the glycosylated form is approximately 60-70 kDa. (PMID: 31142270, PMID: 31819036)

Publikationen

Species	Application	Title
human	IHC	Proc Natl Acad Sci U S A; CRLX101 nanoparticles localize in human tumors and not in adjacent, nonneoplastic tissue after intravenous dosing.; Authors - Andrew J Clark
human	WB	Cell Commun Signal; KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma; Authors - Mao Shen
human	WB	Biomed Pharmacother; Para-toluenesulfonamide, a novel potent carbonic anhydrase inhibitor, improves hypoxia-induced metastatic breast cancer cell viability and prevents resistance to αPD-1 therapy in triple-negative breast cancer; Authors - Hsin-Yuan Chen
mouse,human	WB,IHC,IF	Oxid Med Cell Longev; Carbonic Anhydrase IX Controls Vulnerability to Ferroptosis in Gefitinib-Resistant Lung Cancer; Authors - Chen Zhang
mouse	IHC	Oxid Med Cell Longev; Targeting the Ang2/Tie2 Axis with Tanshinone IIA Elicits Vascular Normalization in Ischemic Injury and Colon Cancer.; Authors - Wei Zou
mouse	IHC,IF	Eur J Nucl Med Mol Imaging; In vivo three-dimensional evaluation of tumour hypoxia in nasopharyngeal carcinomas using FMT-CT and MSOT.; Authors - Wenhui Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aline Seiko (Verified Customer) (05-12-2025)

It was observed a 70kDa and 25kDa isoform.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Renal cell carcinoma

FH

Maelle (Verified Customer) (01-03-2025)

Lost efficacy after freeze-defreze even if it is in glycerol

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1.250eme
• Cell Tissue Type: liver metastasis of colorectal cancer

FH

Josh (Verified Customer) (10-28-2021)

15 ug of human clear cell renal cell carcinoma cell line RCC4 was resolved on 15% Tris/Glycine and transferred to PVDF membrane. Membrane was blocked in blocking buffer (2% BSA in TBS/0.1% Tween-20) for 1hr at room temperature, followed by overnight incubation with anti-CA9 (1:1000) in blocking buffer at 4oC. After 1hr incubation with anti-Rabbit secondary antibody, membrane was imaged with ECL. Expected band was detected along with several non-specific bands by western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: clear cell renal cell carcinoma cell line RCC4

Carbonic Anhydrase IX/CA9 Antibody Western Blot validation (1:1000 dilution) in clear cell renal cell carcinoma cell line RCC4 (Cat no:11071-1-AP)

FH

K (Verified Customer) (08-03-2021)

total cell lysate (50 ug of human HCC cell lines) was resolved on 10% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% BSA in TBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CA9 in blocking buffer (1:500) at 40C overnight. After 1h incubation with appropriate secondary antibody (anti-Rabbit 1:5000) membranes were imaged with ECL and expected band was detected.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HCC cells

Carbonic Anhydrase IX/CA9 Antibody Western Blot validation (1:500 dilution) in HCC cells (Cat no:11071-1-AP)

FH

Paulo (Verified Customer) (03-04-2019)

Total cell lysate (50 ug of human ccRCC cell lines) was resolved on 10% Bis-Tris gel and transferred to PVDF membrane. Membrane was incubated in blocking buffer (5% milk in PBS/0.1% Tween-20) for 1h. Membrane was incubated with anti-CAIX in blocking buffer (1:250) at 4C overnight. After 1h incubation with appropriate secondary antibody (DAKO anti-Rabbit 1:10000) membranes were imaged with ECL and expected band was detected

• Applications: Western Blot,
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Human kidney cancer cells